Institute of Life Sciences 3 articles published in JoVE Biology Quantification of Circular RNAs Using Digital Droplet PCR Arundhati Das*1,2, Debojyoti Das*1,2, Amaresh C. Panda1 1Institute of Life Sciences, 2School of Biotechnology, KIIT University This protocol describes the detailed method of digital droplet PCR (dd-PCR) for precise quantification of circular RNA (circRNA) levels in cells using divergent primers. Cancer Research Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors Amlan P. Mohapatra*1,3, Deepti Parida*1,3, Debasish Mohapatra1,4, Usharani Nayak2,3, Rajeeb K. Swain2, Shantibhusan Senapati1 1Tumor microenvironment and animal models lab, Institute of Life Sciences, 2Vascular Biology lab, Institute of Life Sciences, 3Regional Centre for Biotechnology, 4School of Biotechnology, KIIT University The zebrafish has recently been exploited as a model to validate potential radiation modifiers. The present protocol describes the detailed steps to use zebrafish embryos for radiation-based screening experiments and some observational approaches to evaluate the effect of different treatments and radiation. Biochemistry In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays Ruchir C. Bobde*1,2, Ketul Saharan*1,2, Somanath Baral1,3, Surajit Gandhi1,2, Archana Samal1,2, Rajivgandhi Sundaram1, Ashish Kumar1,4, Ajit K. Singh1,5, Aritreyee Datta1, Dileep Vasudevan1 1Institute of Life Sciences, 2Regional Centre for Biotechnology, 3School of Biotechnology, KIIT University, 4Department of Molecular Biophysics and Biochemistry, Yale University, 5Department of Pharmacology, University of Vermont College of Medicine This protocol describes a battery of methods that includes analytical size-exclusion chromatography to study histone chaperone oligomerization and stability, pull-down assay to unravel histone chaperone-histone interactions, AUC to analyze the stoichiometry of the protein complexes, and histone chaperoning assay to functionally characterize a putative histone chaperone in vitro.
