Sambalpur University 1 article published in JoVE Biochemistry Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors Pushpanjali Soppina1,2, Dipeshwari J. Shewale1, Pradeep K. Naik2, Virupakshi Soppina1 1Discipline of Biological Engineering, Indian Institute of Technology Gandhinagar, 2Department of Biotechnology and Bioinformatics, Sambalpur University This study details purification of KIF1A(1-393LZ), a member of kinesin-3 family, using Sf9-baculovirus expression system. In vitro single-molecule and multi-motor gliding analysis of these purified motors exhibited robust motility properties comparable to motors from mammalian cell lysate. Thus, Sf9-baculovirus system is amenable to express and purify motor protein of interest.