SOKENDAI (The Graduate University for Advanced Studies) 5 articles published in JoVE Neuroscience In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice Hidenobu Mizuno1,2,3, Shingo Nakazawa2,3, Takuji Iwasato2,3 1International Research Center for Medical Sciences (IRCMS), Kumamoto University, 2Division of Neurogenetics, National Institute of Genetics, 3Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies) We present an in vivo two-photon imaging protocol for imaging the cerebral cortex of neonatal mice. This method is suitable for analyzing the developmental dynamics of cortical neurons, the molecular mechanisms that control the neuronal dynamics, and the changes in neuronal dynamics in disease models. Behavior Measuring Active and Passive Tameness Separately in Mice Hiromichi Nagayama1,2, Yuki Matsumoto1,2, Akira Tanave1, Motoko Nihei1, Tatsuhiko Goto3, Tsuyoshi Koide1,2 1Mouse Genomics Resource Laboratory, National Institute of Genetics, 2Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), 3Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine Tameness in animals includes a reduction of avoidance responses to humans (passive tameness) and an increase in active approaches to humans (active tameness). Here, we describe detailed protocols for three behavioral tests (active tameness, passive tameness, and stay-on-hand tests) to separately measure the active and passive tameness of mice. Neuroscience Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae Akira Muto1, Koichi Kawakami1 1Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies) Here, we present a protocol to ablate a genetically labeled subpopulation of neurons by a two-photon laser from Zebrafish larvae. Biochemistry An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis Masatake Kanai1, Shoji Mano1,2, Mikio Nishimura3 1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, 2Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology, National Institute for Basic Biology We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds. Behavior Neuro-rehabilitation Approach for Sudden Sensorineural Hearing Loss Kenichi Sekiya1,2, Munehisa Fukushima3, Henning Teismann4,5, Lothar Lagemann4, Ryusuke Kakigi1,6, Christo Pantev4, Hidehiko Okamoto1,4,6 1Department of Integrative Physiology, National Institute for Physiological Sciences, 2Department of Otolaryngology, Head and Neck Surgery, Nagoya City University Graduate School of Medical Sciences and Medical School, 3Department of Otorhinolaryngology, Kansai Rosai Hospital, 4Institute for Biomagnetism and Biosignalanalysis, University of Muenster, 5Institute for Epidemiology and Social Medicine, University of Muenster, 6Sokendai Graduate University for Advanced Studies This article reports the development of a neuro-rehabilitation approach, "constraint-induced sound therapy (CIST)" for sudden sensorineural hearing loss. The aim of CIST is to prevent maladaptive cortical reorganization by using an enriched acoustic environment. CIST represents a safe, easy, inexpensive, and effective approach to treat sudden sensorineural hearing loss.
