Kato Acoustics Consulting Office 1 article published in JoVE Behavior Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test Akari Asaba1, Masahiro Kato3, Nobuyoshi Koshida2, Takefumi Kikusui1 1School of Veterinary Medicine, Azabu University, 2Division of Electric and Information Engineering, Tokyo University of Agriculture and Technology, 3Kato Acoustics Consulting Office Ultrasonic vocalizations (USVs) in mice differ depending on age, sex, condition, and genetic background. Using two ultrasound emitters broadcasting simultaneously in different locations, this two-choice test can evaluate murine recognition and preference responses to different characteristics of USVs.