Japan Society for the Promotion of Science 3 articles published in JoVE Neuroscience A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales Kenta Yamauchi1,2, Shinichiro Okamoto1,2,3, Megumu Takahashi1,2,4,5, Masato Koike2,3, Takahiro Furuta6, Hiroyuki Hioki1,2,7 1Department of Neuroanatomy, Juntendo University Graduate School of Medicine, 2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 3Advanced Research Institute for Health Sciences, Juntendo University, 4Department of Neuroscience, Graduate School of Medicine, Kyoto University, 5Japan Society for the Promotion of Science, 6Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, 7Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine The protocol provides a detailed method of neuronal imaging in brain slice using a tissue clearing method, ScaleSF. The protocol includes brain tissue preparation, tissue clarification, handling of cleared slices and confocal laser scanning microscopy imaging of neuronal structures from mesoscopic to microscopic levels. Medicine Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System Kazuma Sekiba1,2, Motoyuki Otsuka1, Kazuhiko Koike1 1Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, 2Research Fellow of Japan Society for the Promotion of Science Here, we present a method for screening anti-hepatitis B viral agents that inhibit the HBx-DDB1 interaction using a split luciferase assay system. This system allows easy detection of protein-protein interactions and is suitable for identifying inhibitors of such interactions. Behavior A Task for Assessing the Impact of a Partner on the Speed and Accuracy of Motor Performance in Rats Yayoi Sekiguchi1,2, Toshimichi Hata3 1Graduate School of Psychology, Doshisha University, 2Japan Society for the Promotion of Science, 3Faculty of Psychology, Doshisha University A procedure to measure the speed and accuracy of rats’ motor performance in a social condition is described. The protocol enables us to investigate the effect of the mere presence of others on speed and accuracy of motor performance in one experiment.
