Yamaguchi University of Medicine 1 article published in JoVE Bioengineering Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype Kinya Matsuo1, Britta Engelhardt2, Hideaki Nishihara3 1Graduate School of Medicine, Yamaguchi University, 2Theodor Kocher Institute, University of Bern, 3Department of Neurotherapeutics, Yamaguchi University of Medicine Here, we describe a protocol, the extended endothelial cell culture method (EECM), that allows differentiation of pluripotent stem cells to brain microvascular endothelial cell (BMEC)-like cells. These cells show endothelial cell adhesion molecule expression and are thus a human blood-brain barrier model suitable to study immune cell interactions in vitro.