Korea Brain Research Institute 2 articles published in JoVE Biochemistry Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy Minkyo Jung1, Ji Young Mun1 1Neural Circuits Research Group, Korea Brain Research Institute We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy. Biology Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy Min Kyo Jung1, Ji Young Mun2 1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences, Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network, Korea Brain Research Institute This protocol describes the various techniques necessary for transmission electron microscopy including negative staining, ultrathin sectioning for detailed structure, and immuno-gold labelling to determine the positions of specific proteins in exosomes.
