Korea University Anam Hospital 1 article published in JoVE Bioengineering Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells Dae Hwan Kim*1, Long-Hui Cui*2, Ha-Rim Seo2, Hyung Joon Joo2, Seung-Cheol Choi2, Do-Sun Lim2, Kyu Back Lee1 1School of Biomedical Engineering, Korea University, 2Department of Cardiology, Korea University Anam Hospital Here, we present a protocol for the fabrication of gradient nanopattern plates via thermal nanoimprinting and the method of screening responses of human endothelial progenitor cells to the nanostructures. By using the described technology, it is possible to produce a scaffold that can manipulate cell behavior by physical stimuli.
