Singapore National Eye Centre 2 articles published in JoVE Bioengineering Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy Su Yin Chaw1,2, Tina Tzee Ling Wong3,4, Subbu Venkatraman2,5, Ann-Marie Chacko1 1Laboratory for Translational and Molecular Imaging, Cancer and Stem Cell Biology Programme, Duke-NUS Medical School, 2School of Materials Science and Engineering, Nanyang Technological University, 3Singapore National Eye Centre, 4Singapore Eye Research Institute, 5Material Science & Engineering, National University of Singapore We present a protocol for the use of fiberoptic confocal laser microendoscopy (CLM) to non-invasively study the spatio-temporal distribution of liposomes in the eye after subconjunctival injection. Medicine A Step by Step Protocol for Subretinal Surgery in Rabbits Sami Al-Nawaiseh1, Fabian Thieltges1, Zengping Liu1,2, Claudine Strack1, Ralf Brinken1, Norbert Braun3, Marc Wolschendorf3, Arvydas Maminishkis5, Nicole Eter4, Boris V. Stanzel1,6 1Department of Ophthalmology, University of Bonn, 2Department of Ophthalmology, National University of Singapore, 3Geuder AG, 4Department of Ophthalmology, University of Münster, 5Section on Epithelial and Retinal Physiology and Disease, National Eye Institute/National Institutes of Health, 6Surgical Retina Department, Singapore National Eye Centre Retinal pigment epithelium (RPE) replacement strategies and gene-based therapy are considered for several retinal degenerative conditions. For clinical translation, large eye animal models are required to study surgical techniques applicable in patients. Here we present a rabbit model for subretinal surgery geared towards RPE transplantation, which is versatile and cost-efficient.
