Institute of Medical Biology, A*STAR 1 article published in JoVE Bioengineering Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding Paula Benny1,2, Cedric Badowski2, E. Birgitte Lane2, Michael Raghunath1,2,3 1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.
