Institute of Molecular and Cell Biology 1 article published in JoVE Biology Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture Qianfan Bai*1, Zhiqiang Bai*1, Shaohai Xu3, Lei Sun1,2 1Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 2Institute of Molecular and Cell Biology, Singapore, 3Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.
