Genome Institute of Singapore 1 article published in JoVE Immunology and Infection Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis EnJun Yang1, Jacqueline L.Y. Chee1, Suhanya Duraiswamy2, Siyi Chen3, Kristin Lees3, Swaine L. Chen1,3 1Infectious Diseases Group, Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering, National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore This protocol describes a simple method of isolating single, infected-bladder epithelial cells from a murine model of urinary tract infection.
