National Taipei University of Technology 4 articles published in JoVE Medicine Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium Chun Hsiung*1,2, Yu-Ting Liu*1,2, Chen-Ying Su3, Chung-Chuan Hsiung4,5, Kuo-Hsuan Hung2,6,7, Lung-Kun Yeh2,6 1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo. Bioengineering An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation Hua-De Gao1,2, Pounraj Thanasekaran1, Tzu-Ho Chen1,2, Yu-Hsu Chang3, Yu-Ju Chen1,2, Hsien-Ming Lee1 1Institute of Chemistry, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Materials and Mineral Resources Engineering, National Taipei University of Technology In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol. Biochemistry Preparation of Homogeneous MALDI Samples for Quantitative Applications Yu-Meng Ou1,2, Chien-Wei Tsao1,3, Yin-Hung Lai1, Hsun Lee1, Huan-Tsung Chang2, Yi-Sheng Wang1 1Genomic Research Center, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology A protocol for reducing spatial heterogeneities of ion signals in MALDI mass spectrometry by regulating substrate temperature during sample drying processes is demonstrated. Engineering Synthesis and Characterization of High c-axis ZnO Thin Film by Plasma Enhanced Chemical Vapor Deposition System and its UV Photodetector Application Chung-Hua Chao1, Da-Hua Wei1 1Institute of Manufacturing Technology and Department of Mechanical Engineering, National Taipei University of Technology (TAIPEI TECH) We offered a method to directly synthesize high c-axis (0002) ZnO thin film by plasma enhanced chemical vapor deposition. The as-synthesized ZnO thin film combined with Pt interdigitated electrode was used as sensing layer for ultraviolet photodetector, showing a high performance through a combination of its good responsivity and reliability.
