National Taiwan University College of Medicine 4 articles published in JoVE Immunology and Infection Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro Yu-Ling Hsiao1, Hans Häcker2, Chien-Kuo Lee1 1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation. Immunology and Infection Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research Yu-Jei Lin1, Tsung-Lin Lee1, Chia-Chi Ku1 1Graduate Institute of Immunology, National Taiwan University College of Medicine This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation. Developmental Biology Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues Yu-Jen Chen1,2, Hui-Yu Liu2, Yun-Tsui Chang2, Ying-Hung Cheng2, Harry J. Mersmann2, Wen-Hung Kuo3, Shih-Torng Ding1,2 1Institute of Biotechnology, National Taiwan University, 2Department of Animal Science and Technology, National Taiwan University, 3Department of Surgery, National Taiwan University Hospital and College of Medicine This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies. Immunology and Infection In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System Shiun Chang1, Li-Mei Pai2,3, Chien-Kuo Lee1 1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Biochemistry, Chang Gung University College of Medicine, 3Molecular Medicine Research Center, Chang Gung University College of Medicine In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.
