Chang Gung Memorial Hospital 7 articles published in JoVE Medicine Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium Kuo Hsuan Hung1,2,3, Lung Kun Yeh1,2 1Department of Ophthalmology, Chang-Gung Memorial Hospital, 2Chang-Gung University College of Medicine, 3Institute of Clinical Medicine, National Yang Ming Chiao Tung University Here, animal models based on mouse and rabbit are developed for mechanical and chemical injury of corneal epithelium to screen new therapeutics and the underlying mechanism. Cancer Research Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres Chun-Chia Cheng1, Po-Jui Hsu4, Zong-Lin Sie1, Fang-Hsin Chen1,2,3 1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells. Medicine Development of a Novel Task-oriented Rehabilitation Program using a Bimanual Exoskeleton Robotic Hand Yi-Mei Chen1, Szu-Shen Lai1, Yu-Cheng Pei2,3,4,5, Chia-Ju Hsieh1, Wei-Han Chang1 1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Taoyuan, 2Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, 3School of Medicine, Chang Gung University, 4Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, 5Healthy Aging Research Center, Chang Gung University This study reports the development of a novel robot-assisted task-oriented program for hand rehabilitation. The developmental process consists of experiments using both healthy subjects and subjects who have had a stroke and suffered from subsequent motor control dysfunction. Bioengineering A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva Yueh-Feng Wu1, Rai-Teng Ye1, Ming-Kai Pan2,3, Sung-Jan Lin*1,3,4,5, Hsin-Yuan Tan*6,7 1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures. Medicine Integration of Brain Tissue Saturation Monitoring in Cardiopulmonary Exercise Testing in Patients with Heart Failure Shu-Chun Huang1,2,3, Carl PC Chen1,3, Tieh-Cheng Fu4, Yu-Jen Chen1,5,6 1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, 2Healthy Aging Research Center, Chang Gung University, 3School of Medicine, College of Medicine, Chang Gung University, 4Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Keelung, 5National Quemoy University, 6Kinmen Hospital, Ministry of Health and Welfare This protocol integrated near-infrared spectroscopy into conventional cardiopulmonary exercise testing to identify the involvement of the cerebral hemodynamic response in exercise intolerance in patients with heart failure. Bioengineering Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells Yen-Li Wang1,2, Adrienne Hong3, Tzung-Hai Yen1,4,5,6, Hsiang-Hsi Hong1,7,8 1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes. Medicine Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography Hui-Wen Cheng*1,2, Sudeshna Fisch*1, Susan Cheng1, Michael Bauer1, Soeun Ngoy1, Yiling Qiu1, Jian Guan1, Shikha Mishra1, Christopher Mbah1, Ronglih Liao1 1Cardiac Muscle Research Labratory, Cardiovascular Division, Brigham and Women’s Hospital, Harvard Medical School, 2Cardiovascular Department, Chang Gung Memorial Hospital Right ventricle (RV) dysfunction is critical to the pathogenesis of cardiovascular disease, yet limited methodologies are available for its evaluation. Recent advances in ultrasound imaging provide a noninvasive and accurate option for longitudinal RV study. Herein, we detail a step-by-step echocardiographic method using a murine model of RV pressure overload.
