National Taiwan University Hospital 5 articles published in JoVE Biochemistry Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis Hui-Lan Chang*1, Kang-Yi Su*1,2, Steven D. Goodman3, Wern-Cherng Cheng2, Liang-In Lin1,2, Ya-Chien Yang1,2, Sui-Yuan Chang1,2, Woei-horng Fang1,2 1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University A non-labeled, non-radio-isotopic method to assay uracil-DNA glycosylase activity was developed using MALDI-TOF mass spectrometry for direct apurinic/apyrimidinic site-containing product analysis. The assay proved to be quite simple, specific, rapid, and easy to use for DNA glycosylase measurement. Bioengineering A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva Yueh-Feng Wu1, Rai-Teng Ye1, Ming-Kai Pan2,3, Sung-Jan Lin*1,3,4,5, Hsin-Yuan Tan*6,7 1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures. Behavior Computerized Adaptive Testing System of Functional Assessment of Stroke Gong-Hong Lin1, Yi-Jing Huang1, Yeh-Tai Chou2, Hsin-Yu Chiang3, Ching-Lin Hsieh1,4,5 1School of Occupational Therapy, College of Medicine, National Taiwan University, 2Research Center for Psychological and Educational Testing, National Taiwan Normal University, 3Department of Occupational Therapy, College of Medicine, Fu Jen Catholic University, 4Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital, 5Department of Occupational Therapy, College of Medical and Health Science, Asia University Here, we present a protocol to develop the computerized adaptive testing system of the functional assessment of stroke (CAT-FAS). The CAT-FAS can simultaneously assess four functions (two motor functions [upper and lower extremities], postural control, and basic activities of daily living) with sufficient reliability and administrative efficiency. Biochemistry Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis Kang-Yi Su*1,2, Steven D. Goodman*3, Hung-Ming Lai1, Rong-Syuan Yen1, Wei-Yao Hu1, Wern-Cherng Cheng2, Liang-In Lin1,2, Ya-Chien Yang1,2, Woei-Horng Fang1,2 1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University A non-labeled, non-radio-isotopic method for DNA polymerase proofreading and a DNA repair assay was developed by using high-resolution MALDI-TOF mass spectrometry and a single nucleotide extension strategy. The assay proved to be very specific, simple, rapid, and easy to perform for proofreading and repair patches shorter than 9-nucleotides. Developmental Biology In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition Wei-Ting Ho1,2, Chien-Chia Su3, Jung-Shen Chang3, Shu-Wen Chang1, Fung-Rong Hu3, Tzuu-Shuh Jou2,4, I-Jong Wang3,4 1Department of Ophthalmology, Far Eastern Memorial Hospital, 2Institute of Clinical Medicine, National Taiwan University, 3Department of Ophthalmology, National Taiwan University Hospital, 4College of Medicine, National Taiwan University A primary culture of bovine corneal endothelial cells was used to investigate the mechanism of corneal endothelial-mesenchymal transition. Furthermore, a rat corneal endothelium cryoinjury model was used to demonstrate corneal endothelial-mesenchymal transition in vivo.
