Mackay Memorial Hospital and Mackay Medical College 3 articles published in JoVE Cancer Research Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres Chun-Chia Cheng1, Po-Jui Hsu4, Zong-Lin Sie1, Fang-Hsin Chen1,2,3 1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells. Behavior Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture Pei-Lu Yi*1, Shuo-Bin Jou*2, Yi-Jou Wu3, Fang-Chia Chang3,4,5 1Department of Sports, Health & Leisure, College of Tourism, Leisure and Sports, Aletheia University, Tainan Campus, 2Department of Neurology, Mackay Memorial Hospital and Mackay Medical College, 3Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, 4Graduate Institute of Brain & Mind Sciences, College of Medicine, National Taiwan University, 5Graduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University This paper demonstrates the performance of acupuncture, epilepsy models, and the analysis of sleep in rodents. The acupuncture procedure and the identification of acupoints are described. Pilocarpine or pentylenetetrazol (PTZ) is used to induce epilepsy. Electrocorticogram (ECoG), electromyogram (EMG), brain temperature, and locomotor activity recordings are employed for sleep analysis. Medicine A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats Hui-Chun Ku1, Shih-Yi Lee2,3, Yuan-Kun Aden Wu4, Kai-Chien Yang1,5, Ming-Jai Su1 1Graduate Institute of Pharmacology, College of Medicine, National Taiwan University, 2Division of Pulmonary and Critical Care Medicine, Mackay Memorial Hospital, 3Mackay Medicine, Nursing and Management College, 4School of Medicine, National Taiwan University, 5Department of Internal Medicine, National Taiwan University Hosptial A rat model of abdominal aortic constriction that induces cardiac hypertrophy and remodeling is described. An efficient, highly-reproducible, and minimally-invasive method is used to provide a simple yet useful platform for research in myocardial hypertrophy and dysfunction.
