Wan Fang Hospital 1 article published in JoVE Cancer Research Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment Chi-Hao Hsiao1,2,3, Ya-Hsu Chiu4, Shao-Chih Chiu4,5, Der-Yang Cho4,6,7, Liang-Ming Lee1,2, Yu-Ching Wen1,2, Jia-Jhe Li4, Ping-Hsiao Shih4 1Department of Urology, Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, 5Graduate Institute of Biomedical Sciences, China Medical University, 6Graduate Institute of Immunology, China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3+CD56+ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.
