RMIT University 6 articles published in JoVE Engineering In Situ Transmission Electron Microscopy with Biasing and Fabrication of Asymmetric Crossbars Based on Mixed-Phased a-VOx Shruti Nirantar*1, Edwin Mayes*2, Sharath Sriram1 1Functional Materials and Microsystems Research Group and the Micro Nano Research Facility, RMIT University, 2RMIT Microscopy and Microanalysis Facility, RMIT University Presented here is a protocol for analyzing nanostructural changes during in situ biasing with transmission electron microscopy (TEM) for a stacked metal-insulator-metal structure. It has significant applications in resistive switching crossbars for the next generation of programmable logic circuits and neuromimicking hardware, to reveal their underlying operation mechanisms and practical applicability. Environment Combining Eye-tracking Data with an Analysis of Video Content from Free-viewing a Video of a Walk in an Urban Park Environment Marco Amati1,2, Chris McCarthy3, Ebadat Ghanbari Parmehr4, Jodi Sita5 1Visiting Professor, Dipartimento di Scienze Agro-Ambientali e Territoriali, Università degli Studi di Bari, 2Centre for Urban Research, Royal Melbourne Institute of Technology (RMIT University), 3School of Software and Electrical Engineering, Swinburne University of Technology, 4Faculty of Civil Engineering, Babol Noshirvani University of Technology, 5School of Science, Australian Catholic University The objective of the protocol is to detail how to collect video data for use in the laboratory; how to record eye-tracking data of participants looking at the data and how to efficiently analyze the content of the videos that they were looking at using a machine learning technique. Immunology and Infection Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions Thomas A. Angelovich1,2, Anna C. Hearps1,3, Anna Maisa1, Theodoros Kelesidis4, Anthony Jaworowski1,3 1Centre for Biomedical Research, Burnet Institute, 2School of Health and Biomedical Sciences, RMIT University, 3Department of Infectious Diseases, Monash University, 4University of California, Los Angeles We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity. Immunology and Infection Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein Shubhendu Sen Roy1, Huy Cong Xuan Nguyen1, Thomas A. Angelovich2,3, Anna C. Hearps2, Diana Huynh1,4, Anthony Jaworowski2,4, Theodoros Kelesidis1 1University of California, Los Angeles, 2Centre for Biomedical Research, Burnet Institute, 3School of Health and Biomedical Sciences, RMIT University, 4Department of Infectious Diseases, Monash University We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease. Engineering The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight Vi Khanh Truong1, Miljan Stefanovic1, Shane Maclaughlin2, Mark Tobin3, Jitraporn Vongsvivut3, Mohammad Al Kobaisi1, Russell J. Crawford4, Elena P. Ivanova1 1School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 2BlueScope Steel Research, 3Infrared Microspectroscopy Beamline, Australian Synchrotron, 4School of Science, College of Science, Engineering and Health, RMIT University Two types of surfaces, polyester-coated steel and polyester coated with a layer of silica nanoparticles, were studied. Both surfaces were exposed to sunlight, which was found to cause substantial changes in the chemistry and nanoscale topography of the surface. Neuroscience A Method for Systematic Electrochemical and Electrophysiological Evaluation of Neural Recording Electrodes Alexander R. Harris1,3, Simeon J. Morgan1,3, Gordon G. Wallace3, Antonio G. Paolini1,3,4 1School of Psychological Science, La Trobe University, 2Intelligent Polymer Research Institute, University of Wollongong, 3ARC Centre of Excellence for Electromaterials Science, 4Health Innovations Research Institute, College of Science, Engineering, and Health, RMIT University Different electrode coatings affect neural recording performance through changes to electrochemical, chemical and mechanical properties. Comparison of electrodes in vitro is relatively simple, however comparison of in vivo response is typically complicated by variations in electrode/neuron distance and between animals. This article provides a robust method to compare neural recording electrodes.
