Monash Medical Centre 3 articles published in JoVE Immunology and Infection Assessing Respiratory Immune Responses to Haemophilus Influenzae Lovisa Dousha1,2, Roleen Sharma1,2, Steven Lim2,3, James Ngui2,4, Ashley M. Buckle5, Paul T. King1,2 1Monash Lung and Sleep, Monash Medical Centre, 2Monash University Department of Medicine, Monash Medical Centre, 3Flow Cytometry Science Technology Platform, Francis Crick Institute, 4Clinical Immunology, Monash Medical Centre, 5Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University Haemophilus influenzae induces inflammation in the respiratory tract. This article will focus on the use of flow cytometry and confocal microscopy to define immune responses by phagocytes and lymphocytes in response to this bacterium. Immunology and Infection Visualizing Macrophage Extracellular Traps Using Confocal Microscopy Roleen Sharma1,2, Kim M. O'Sullivan2, Stephen R. Holdsworth2, Philip G. Bardin1,2, Paul T. King1,2 1Monash Lung and Sleep, Monash Medical Centre, 2Monash University Macrophage extracellular traps are a newly described entity. This article will concentrate on confocal microscopy methods and how they are visualized in vitro and in vivo from lung samples. Biology Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography Rebecca Lim1,2, Marcus J. Zavou1, Phillipa-Louise Milton3, Siow Teng Chan1, Jean L. Tan1, Hayley Dickinson1,2, Sean V. Murphy4, Graham Jenkin1,2, Euan M. Wallace1,2 1The Ritchie Centre, Monash Institute of Medical Research, 2Department of Obstetrics and Gynaecology, Monash Medical Centre, 3Animal Resource Centre, Perth, Australia, 4Wake Forest Institute for Regenerative Medicine The assessment of respiratory physiology has traditionally relied upon techniques, which require restraint or sedation of the animal. Unrestrained whole-body plethysmography, however, provides precise, non-invasive, quantitative analysis of respiratory physiology in animal models. In addition, the technique allows repeated respiratory assessment of mice allowing for longitudinal studies.
