Surgical Diagnostics Pty Ltd. 1 article published in JoVE Biology Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes Amani Alghalayini1,2, Lele Jiang1, Xi Gu3, Guan Heng Yeoh3, Charles G. Cranfield1,2, Victoria Timchenko3, Bruce A. Cornell2,4, Stella M. Valenzuela1,2,5 1School of Life Sciences, University of Technology Sydney, 2ARC Research Hub for Integrated Devices for End-user Analysis at Low-levels (IDEAL), Faculty of Science, University of Technology Sydney, 3School of Mechanical and Manufacturing Engineering, The University of New South Wales, 4Surgical Diagnostics Pty Ltd., 5Institute for Biomedical Materials and Devices, University of Technology Sydney This work outlines a protocol to achieve dynamic, non-invasive monitoring of heat transfer from laser-irradiated gold nanoparticles to tBLMs. The system combines impedance spectroscopy for the real-time measurement of conductance changes across the tBLMs, with a horizontally focused laser beam that drives gold nanoparticle illumination, for heat production.
