Geneva University Hospitals and Faculty of Medicine 4 articles published in JoVE Bioengineering Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases Erika Cosset*1, Manon Locatelli*2, Antoine Marteyn2, Pierre Lescuyer3, Florence Dall Antonia3, Flavio Maurizio Mor4, Olivier Preynat-Seauve5, Luc Stoppini4, Vannary Tieng2 1Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, 2Department of Pathology and Immunology, University Medical Center, University of Geneva, 3Laboratory of Toxicology and Therapeutic Drug Monitoring, Geneva University Hospitals, 4Tissue Engineering Laboratory, Hepia/HES-SO, University of Applied Sciences Western Switzerland, 5Laboratory of Experimental cell therapy, Department of Diagnostics, Geneva University Hospitals This study introduces and describes protocols to derive two specific human neural organoids as a relevant and accurate model for studying 1) human glioblastoma development within human neural organoids exclusively in humans and 2) neuron dopaminergic differentiation generating a three-dimensional organoid. Medicine Surgical Training for the Implantation of Neocortical Microelectrode Arrays Using a Formaldehyde-fixed Human Cadaver Model Pierre Mégevand1,2, Alain Woodtli1, Aude Yulzari1, G. Rees Cosgrove3, Shahan Momjian4, Bojan V. Stimec5, Marco V. Corniola4, Jean H. D. Fasel5 1Wyss Center for Bio and Neuroengineering, Geneva, 2Division of Neurology, Department of Clinical Neuroscience, Geneva University Hospitals, 3 We designed a procedure in which a formaldehyde-fixed human cadaver is used to assist neurosurgeons in training for the implantation of microelectrode arrays into the neocortex of the human brain. Developmental Biology Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement Thomas Laumonier*1, Stéphane Koenig*2, Sophie Saüc2,3, Maud Frieden3 1Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences, Geneva Medical Center, 3Department of Cell Physiology and Metabolism, Geneva Medical Center Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling. Medicine Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation Filippo Molica1, Séverine Nolli2, Pierre Fontana2, Brenda Renata Kwak1 1Department of Pathology and Immunology, University of Geneva, 2Division of Angiology and Haemostasis & Geneva Platelet Group, Geneva University Hospitals We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.
