Neurocenter of Southern Switzerland 1 article published in JoVE Neuroscience Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay Elena Vacchi1, Sandra Pinton1, Alain Kaelin-Lang1,2,3,4, Giorgia Melli1,2 1Laboratory for Biomedical Neurosciences, Neurocenter of Southern Switzerland, 2Neurology Department, Neurocenter of Southern Switzerland, 3Department of Neurology, Inselspital, Bern University Hospital, University of Bern, 4Faculty of Biomedical Sciences, Università della Svizzera Italiana Here, we present a protocol for a free-floating indirect immunofluorescence assay on skin biopsy sections that allows for the identification of disease specific conformation variants of alpha synuclein involved in Parkinson disease and multiple proteins of the peripheral nervous system.
