University Hospital Kralovske Vinohrady 1 article published in JoVE Biology Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells Moustafa Elkalaf1,2, Karolína Vaněčková1,2, Pavla Staňková1, Zuzana Červinková1, Jan Polák*2,3, Otto Kučera*1 1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady In this work, we describe a modified protocol to test mitochondrial respiratory substrate flux using recombinant perfringolysin O in combination with microplate-based respirometry. With this protocol, we show how metformin affects mitochondrial respiration of two different tumor cell lines.
