Universitatsklinikum Tubingen 4 articles published in JoVE Bioengineering Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA Tatjana Michel1, Antonia Link1, Meike-Kristin Abraham1,2, Christian Schlensak1, Karlheinz Peter2,3, Hans-Peter Wendel1, Xiaowei Wang2,3, Stefanie Krajewski1 1Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, 2Atherothrombosis and Vascular Biology, Baker Heart & Diabetes Institute, 3Department of Medicine, Monash University Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA. Medicine Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin Bernhard N. Bohnert1,2,3, Ferruh Artunc1,2,3 1Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Disease, Nephrology and Clinical Chemistry, University Hospital Tübingen, 2Institute of Diabetes Research and Metabolic Diseases (IDM) of the Helmholtz Center Munich, University Tübingen, 3German Center for Diabetes Research (DZD), University Tübingen Here, we describe the induction of experimental nephrotic syndrome in 129S1/SvImJ mice by rapid retrobulbar injection of doxorubicin. We also treat nephrotic mice with sustained release pellets containing aprotinin to inhibit urinary serine protease activity and prevent sodium retention. Medicine Cardiopulmonary Bypass in a Mouse Model: A Novel Approach Nodir Madrahimov1, Ruslan Natanov1, Erin C. Boyle1, Tobias Goecke1, Ann-Kathrin Knöfel1, Valentyna Irkha1, Anna Solovieva2, Klaus Höffler1, Ulrich Maus3, Christian Kühn1, Issam Ismail1, Gregor Warnecke1, Malakh-Lal Shrestha1, Serghei Cebotari*1, Axel Haverich*1 1Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, 2Department of Hematology, Oncology, Immunology, Rheumatology, and Pulmonology, University Hospital Tuebingen, 3Department of Pneumology, Hannover Medical School This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage. Behavior How to Study Placebo Responses in Motion Sickness with a Rotation Chair Paradigm in Healthy Participants Katja Weimer1, Björn Horing2, Eric R. Muth2, Paul Enck1 1Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, 2Department of Psychology, Clemson University An experimental rotation chair paradigm is used to investigate whether a placebo intervention on motion sickness affects subjective outcome measures only or also affects behavioral and objective measures in a balanced placebo design.
