Munich Heart Alliance 2 articles published in JoVE Medicine Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse Ruibing Xia1,2,3, Julia Vlcek1,2, Julia Bauer1,2,3, Stefan Kääb1,3, Hellen Ishikawa-Ankerhold1,2, Dominic Adam van den Heuvel1,2, Christian Schulz1,2,3, Steffen Massberg1,2,3, Sebastian Clauss1,2,3 1University Hospital Munich, Department of Medicine I, Ludwig Maximilians University (LMU) Munich, 2Institute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig Maximilians University (LMU) Munich, 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance We provide a step-by-step protocol for whole-mount immunofluorescence staining of the sinoatrial node (SAN) and atrioventricular node (AVN) in murine hearts. Medicine Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery Christoph Emontzpohl1,2, David Simons3, Sandra Kraemer4, Andreas Goetzenich4, Gernot Marx1, Jürgen Bernhagen5,6, Christian Stoppe1 1Department of Intensive Care Medicine, University Hospital Aachen, 2Institute of Biochemistry and Molecular Biology, University Hospital Aachen, 3Department of Radiology, German Cancer Research Center, 4Department of Thoracic and Cardiovascular Surgery, University Hospital Aachen, 5Department of Vascular Biology, Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, 6Deutsches Zentrum für Herz-/Kreislaufkrankheiten (DZHK), Munich Heart Alliance Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
