LMU Munich 8 articles published in JoVE Medicine Microdissection and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins Hannes E. Villgrater*1,2,3, Ruibing Xia*1,2,3, Aparna Sharma Chivukula1,2,3, Philipp Tomsits*1,2,3, Sebastian Clauss*1,2,3,4 1University Hospital Munich, Department of Medicine I, Ludwig-Maximilians-University Munich (LMU), 2Walter Brendel Center of Experimental Medicine (WBex), LMU Munich, 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 4Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich This protocol demonstrates microscopy-guided isolation and immunofluorescence staining of murine pulmonary veins. We prepare tissue samples containing the left atrium, pulmonary veins, and the corresponding lungs and stain them for cardiac Troponin T and Connexin 43. Medicine Preparation of Human Myocardial Tissue for Long-Term Cultivation Jules Hamers1,2, Payel Sen1,2, Daphne Merkus1,2,3, Thomas Seidel4,5, Kun Lu1,6, Andreas Dendorfer1,2 1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely. Neuroscience Modeling Stroke in Mice: Transient Middle Cerebral Artery Occlusion via the External Carotid Artery Gemma Llovera1, Alba Simats1, Arthur Liesz1,2 1Institute for Stroke and Dementia Research, LMU Munich, 2Munich Cluster for Systems Neurology (SyNergy) Different models of middle cerebral artery occlusion (MCAo) are used in experimental stroke research. Here, an experimental stroke model of transient MCAo via the external carotid artery (ECA) is described, which aims to mimic human stroke, in which the cerebrovascular thrombus is removed due to spontaneous clot lysis or therapy. Neuroscience Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis Gemma Llovera1, Kelsey Pinkham1, Arthur Liesz1,2 1Institute for Stroke and Dementia Research, LMU Munich, 2Munich Cluster for Systems Neurology (SyNergy) Described here is the photothrombotic stroke model, where a stroke is produced through the intact skull by inducing permanent microvascular occlusion using laser illumination after administration of a photosensitive dye. Chemistry 18F-Labeling of Radiotracers Functionalized with a Silicon Fluoride Acceptor (SiFA) for Positron Emission Tomography David Connolly*1, Justin J. Bailey*1, Harun Ilhan2,3, Peter Bartenstein2,3, Carmen Wängler4, Björn Wängler5, Melinda Wuest1, Frank Wuest1, Ralf Schirrmacher1 1Department of Oncology, University of Alberta, 2Department of Nuclear Medicine, University Hospital of Munich, 3ENETS Centre of Excellence, Interdisciplinary Center of Neuroendocrine Tumors of the GastroEnteroPancreatic System, LMU Munich, 4Biomedical Chemistry, Department of Clinical Radiology and Nuclear Medicine, Medical Faculty Mannheim of Heidelberg University, 5Molecular Imaging and Radiochemistry, Department of Clinical Radiology and Nuclear Medicine, Medical Faculty Mannheim of Heidelberg University The synthesis of fluorine-18 (18F) labeled radiopharmaceuticals for positron emission tomography typically requires months of experience. When incorporated into a radiotracer, the silicon-fluoride acceptor (SiFA) motif enables a simple 18F-labeling protocol that is independent of costly equipment and preparatory training, while reducing precursor quantity needed and utilizing milder reaction conditions. Behavior Brain Infarct Segmentation and Registration on MRI or CT for Lesion-symptom Mapping J. Matthijs Biesbroek1, Hugo J. Kuijf2, Nick A. Weaver1, Lei Zhao3, Marco Duering4, Meta VCI Map Consortium, Geert Jan Biessels1 1Department of Neurology and Neurosurgery, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, 2Image Sciences Institute, University Medical Center Utrecht, 3BrainNow Research Institute, 4Institute for Stroke and Dementia Research, University Hospital, LMU Munich Provided here is a practical tutorial for an open-access, standardized image processing pipeline for the purpose of lesion-symptom mapping. A step-by-step walkthrough is provided for each processing step, from manual infarct segmentation on CT/MRI to subsequent registration to standard space, along with practical recommendations and illustrations with exemplary cases. Medicine Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models Andreas Blutke1, Rüdiger Wanke1 1Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, LMU Munich The practical application and performance of methods for generation of representative tissue samples of porcine animal models for a broad spectrum of downstream analyses in biobank projects are demonstrated, including volumetry, systematic random sampling, and differential processing of tissue samples for qualitative and quantitative morphologic and molecular analyses types. Medicine In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in Menière's Disease Ears and Normal Hearing Ears Wilhelm H. Flatz1, Annika Henneberger2, Maximilian F. Reiser1, Robert Gürkov3, Birgit Ertl-Wagner1 1Department of Radiology, University Hospital, LMU Munich, 2Department of Otorhinolaryngology - Head and Neck Surgery, SLK-Kliniken Heilbronn GmbH, 3Department of Otorhinolaryngology Head and Neck Surgery, Ludwig-Maximilians-University Hospital Munich, German Centre for Vertigo and Balance Disorder To evaluate morphological changes of cranial nerves such as loss of neural structures or swelling of cranial nerves in Menière's Disease (MD) or in healthy persons in vivo, a protocol of evaluation has been developed using magnetic resonance imaging (MRI). Additional MRI-based confirmation of MD was performed.
