University Clinic of Heidelberg 1 article published in JoVE Immunology and Infection Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy Xenia Ficht*1, Flavian Thelen*1, Bettina Stolp*1,2, Jens V. Stein1 1Theodor-Kocher Institute, University of Bern, 2Center for Infectious Diseases, Integrative Virology, University Clinic of Heidelberg We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.
