Justus Liebig University Giessen 7 articles published in JoVE Immunology and Infection Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells Fabian Dieter1, Jendrik Grube1, Tim Birkenhauer1, Alice Quentin1, Gunter P. Eckert1 1Biomedical Research Center, Institute of Nutritional Sciences, Justus-Liebig-University of Giessen Isolated peripheral blood mononuclear cells can be used for the analysis of immune functions and disorders, metabolic diseases, or mitochondrial functions. In this work, we describe a standardized method for the preparation of PBMCs from whole blood and the subsequent cryopreservation. Cryopreservation makes this time and place independent. Biochemistry In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines Alice Sosic1, Cristina Dal Lago1, Richard Göttlich2, Barbara Gatto1 1Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 2Institute of Organic Chemistry, Justus Liebig University Giessen Bis-3-chloropiperidines (B-CePs) are useful chemical probes to identify and characterize G-quadruplex structures in DNA templates in vitro. This protocol details the procedure to perform probing reactions with B-CePs and to resolve reaction products by high-resolution polyacrylamide gel electrophoresis. Behavior Estimation of Contact Regions Between Hands and Objects During Human Multi-Digit Grasping Frieder Hartmann1, Guido Maiello1, Constantin A. Rothkopf2, Roland W. Fleming1,3 1Department of Experimental Psychology, Justus Liebig University Giessen, 2Institute of Psychology & Centre for Cognitive Science, Technical University of Darmstadt, 3Centre for Mind, Brain and Behaviour (CMBB), University of Marburg and Justus Liebig University Giessen When we grasp an object, multiple regions of the fingers and hand typically make contact with the object's surface. Reconstructing such contact regions is challenging. Here, we present a method for approximately estimating the contact regions by combining marker-based motion capture with existing deep learning-based hand mesh reconstruction. Chemistry Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry Karolin Bomhardt*1, Pascal Schneider*1, André Portz1, Christoph R. Gebhardt2, Michael Dürr1 1Institute of Applied Physics and Center for Materials Research (LaMa), Justus Liebig University Giessen, 2Bruker Daltonik GmbH Neutral SO2 clusters of low kinetic energy (< 0.8 eV/constituent) are used to desorb complex surface molecules such as peptides or lipids for further analysis by means of mass spectrometry using an ion trap mass spectrometer. No special sample preparation is required, and real-time observation of reactions is possible. Behavior Errors as a Means of Reducing Impulsive Food Choice Manuela Sellitto1,2, Giuseppe di Pellegrino2 1Department of Biological Psychology, Justus-Liebig-Universität Giessen, 2Dipartimento di Psicologia, Università di Bologna Giving in to temptation of tasty food may result in long-term overweight problems. This protocol describes how to reduce imprudent preference for edible commodities during hypothetical intertemporal choices in women by associating them with errors. Immunology and Infection Isolation of Sertoli Cells and Peritubular Cells from Rat Testes Sudhanshu Bhushan1, Ferial Aslani1, Zhengguo Zhang1, Tim Sebastian1, Hans-Peter Elsässer2, Jörg Klug1 1Institut für Anatomie und Zellbiologie, Justus-Liebig-Universität Gießen, 2Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes. Medicine Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices Renate Paddenberg1, Petra Mermer1, Anna Goldenberg1, Wolfgang Kummer1 1Institute of Anatomy and Cell Biology, Justus-Liebig-University Hypoxic pulmonary vasoconstriction (HPV) is an important physiological phenomenon by which at alveolar hypoxia lung perfusion is matched to ventilation. The major vascular segment contributing to HPV is the intra-acinar artery. Here, we describe our protocol for the analysis of HPV of murine pulmonary vessels with diameters of 20-100 μm.
