Jena University Hospital 12 articles published in JoVE Neuroscience Manual Segmentation of the Human Choroid Plexus Using Brain MRI Deepthi Bannai*1,2, Yuan Cao*3,4, Matcheri Keshavan1,2, Martin Reuter5,6,7, Paulo Lizano1,2,8 1Department of Psychiatry, Beth Israel Deaconess Medical Center, 2Division of Translational Neuroscience, Beth Israel Deaconess Medical Center, 3Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, 4Department of Psychiatry and Psychotherapy, Jena University Hospital, 5AI in Medical Imaging, German Center for Neurodegenerative Diseases (DZNE), 6A. A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, 7Department of Radiology, Harvard Medical School, 8Department of Psychiatry, Harvard Medical School Despite the crucial role of the choroid plexus in the brain, neuroimaging studies of this structure are scarce due to the lack of reliable automated segmentation tools. The present protocol aims to ensure gold-standard manual segmentation of the choroid plexus that can inform future neuroimaging studies. Engineering Normothermic Ex Vivo Liver Machine Perfusion in Mouse Haotian Chen1, Olaf Dirsch2, Mohamed Albadry1,3, Paz Hernandez Ana1, Uta Dahmen1 1Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Institute of Pathology, Klinikum Chemnitz GmbH, 3Department of Pathology, Faculty of Veterinary Medicine, Menoufia University A normothermic ex vivo liver perfusion (NEVLP) system was created for mouse livers. This system requires experience in microsurgery but allows for reproducible perfusion results. The ability to utilize mouse livers facilitates the investigation of molecular pathways to identify novel perfusate additives and enables the execution of experiments focused on organ repair. Medicine Closure of a Patent Foramen Ovale (PFO): An Intervention Sequence Pawel Aftanski1, Aurel Maloku1, Gudrun Dannberg1, Ali Hamadanchi1, Albrecht Günther2, Paul Christian Schulze1, Sven Möbius-Winkler1 1Department of Internal Medicine I, Division of Cardiology, Angiology, and Intensive Medical Care, University Hospital Jena, 2Department of Neurology, University Hospital Jena The closure of a patent foramen ovale (PFO) is a catheter-based intervention to prevent PFO-associated stroke. The PFO-occlusion device is advanced through the femoral vein and deployed across the interatrial septum using transesophageal echocardiography (TEE) and fluoroscopic guidance. The following protocol provides a step-by-step guide for the PFO-closure intervention using a double-disc device. Immunology and Infection Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry Susann Hartung1,2, Christopher Rauh2, Sarah Böttcher1, Mai Thi Ngoc Hoang1,2, Susanne Jahreis1,2, Silke Rummler3, Andreas Hochhaus2, Marie von Lilienfeld-Toal1,2 1Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology, Jena University Hospital, 3Institute for Transfusion Medicine, Jena University Hospital This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes. Bioengineering A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat An Wang1, Isabel Jank1, Weiwei Wei1, Claudia Schindler1, Uta Dahmen1 1Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, University Hospital Jena We establish a novel surgical technique for an in vivo single liver lobe perfusion model in rat as a prerequisite for further studying in vivo partial liver engineering in the future. Medicine Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice Chichi Xie1, Weiwei Wei1, Andrea Schenk3, Lars Ole Schwen3, Sara Zafarnia2, Michael Schwier3, Felix Gremse2, Isabel Jank1, Olaf Dirsch4, Uta Dahmen1 1Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Experimental Molecular Imaging, RWTH Aachen University, 3Fraunhofer Institute for Medical Image Computing MEVIS, 4Institute of Pathology, Klinikum Chemnitz gGmbH Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections. Chemistry Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue Julia Greiser1, Tobias Niksch1, Wolfgang Weigand2, Martin Freesmeyer1 1Clinic of Nuclear Medicine, University Hospital Jena, 2Friedrich Schiller University A procedure for the isolation of EOB-DTPA and subsequent complexation with natural Ga(III) and 68Ga is presented herein, as well as a thorough analysis of all compounds and investigations on labeling efficiency, in vitro stability and the n-octanol/water distribution coefficient of the radiolabeled complex. Neuroscience Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis Anne Ansorg*1, Katja Bornkessel*1, Otto W. Witte1, Anja Urbach1 1Hans Berger Department of Neurology, Jena University Hospital This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable. Bioengineering Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging Felista L. Tansi*1, Ronny Rüger*2, Markus Rabenhold2, Frank Steiniger3, Alfred Fahr2, Ingrid Hilger1 1Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, Jena University Hospital, 2Department of Pharmaceutical Technology, Friedrich-Schiller-University Jena, 3Center for Electron Microscopy, Jena University Hospital The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity. Medicine Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice Chichi Xie1,3, Weiwei Wei1, Tao Zhang1, Olaf Dirsch2, Uta Dahmen1 1Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Institute of Pathology, Jena University Hospital, 3Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University This film demonstrates how to acquire systemic and hepatic hemodynamics in mice. The whole monitoring includes acquisition of vital parameters, systemic blood pressure, central venous pressure, common hepatic artery flow rate, and portal vein pressure as well as the portal flow rate in mice. Neuroscience In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice Stefanie Fischer*1, Christian Engelmann*2, Karl-Heinz Herrmann3, Jürgen R. Reichenbach3, Otto W. Witte1, Falk Weih2, Alexandra Kretz1, Ronny Haenold2 1Hans Berger Department of Neurology, Jena University Hospital, 2Immunology, Leibniz Institute for Age Research, Fritz Lipmann Institute, Jena, 3Institute of Diagnostic and Interventional Radiology, Medical Physics Group, Jena University Hospital This video illustrates a method, using a clinical 3 T scanner, for contrast-enhanced MR imaging of the naïve mouse visual projection and for repetitive and longitudinal in vivo studies of optic nerve degeneration associated with acute optic nerve crush injury and chronic optic nerve degeneration in knock-out mice (p50KO). Neuroscience Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo Benedikt Grünewald1,2, Christian Geis1,2 1Hans Berger Department of Neurology, Jena University Hospital, Jena, Germany, 2Integrated Research and Treatment Center, Center for Sepsis Control and Care (CSCC), Jena University Hospital, Jena, Germany GABAergic presynaptic inhibition is a powerful inhibitory mechanism in the spinal cord important for motor and sensory signal integration in spinal cord networks. Underlying primary afferent depolarization can be measured by recording of dorsal root potentials (DRP). Here we demonstrate a method of in vivo recording of DRP in mice.
