University Hospital Munich 3 articles published in JoVE Chemistry 18F-Labeling of Radiotracers Functionalized with a Silicon Fluoride Acceptor (SiFA) for Positron Emission Tomography David Connolly*1, Justin J. Bailey*1, Harun Ilhan2,3, Peter Bartenstein2,3, Carmen Wängler4, Björn Wängler5, Melinda Wuest1, Frank Wuest1, Ralf Schirrmacher1 1Department of Oncology, University of Alberta, 2Department of Nuclear Medicine, University Hospital of Munich, 3ENETS Centre of Excellence, Interdisciplinary Center of Neuroendocrine Tumors of the GastroEnteroPancreatic System, LMU Munich, 4Biomedical Chemistry, Department of Clinical Radiology and Nuclear Medicine, Medical Faculty Mannheim of Heidelberg University, 5Molecular Imaging and Radiochemistry, Department of Clinical Radiology and Nuclear Medicine, Medical Faculty Mannheim of Heidelberg University The synthesis of fluorine-18 (18F) labeled radiopharmaceuticals for positron emission tomography typically requires months of experience. When incorporated into a radiotracer, the silicon-fluoride acceptor (SiFA) motif enables a simple 18F-labeling protocol that is independent of costly equipment and preparatory training, while reducing precursor quantity needed and utilizing milder reaction conditions. Medicine Diagnosis of Musculus Gastrocnemius Tightness - Key Factors for the Clinical Examination Sebastian F. Baumbach1,2, Mareen Braunstein1,2, Markus Regauer1,2, Wolfgang Böcker2, Hans Polzer1,2 1Foot and Ankle Surgery, Munich University Hospital, 2Department of Trauma Surgery, Munich University Hospital Isolated Musculus gastrocnemius tightness is a common cause for foot and ankle pathologies. Currently no standardized examination procedure exists. This manuscript demonstrates that 20 degree of knee flexion eliminates the restraining effect of the M. gastrocnemius on ankle dorsiflexion and presents a video description of a standardized examination protocol. Medicine Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery Gemma Llovera*1,2, Stefan Roth*1,2, Nikolaus Plesnila1,2, Roland Veltkamp3,4, Arthur Liesz1,2 1Institute for Stroke and Dementia Research, University Hospital Munich, 2Munich Cluster for Systems Neurology (SyNergy), 3Department of Neurology, University Heidelberg, 4Imperial College, Charing Cross Hospital Various murine models of middle cerebral artery occlusion (MCAO) are widely used in experimental brain research. Here, we demonstrate the model of transcranial permanent distal MCAO which produces consistent cortical infarction of a size corresponding to damage imposed by the majority of human ischemic strokes.
