University Eye Hospital Tubingen 2 articles published in JoVE Medicine A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons Daniel A. Wenzel1, Berenike C. Kunzmann2, Nils A. Steinhorst1, Martin S. Spitzer1, Maximilian Schultheiss1 1Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE), 2University Eye Hospital, Center For Ophthalmology, University Hospital Tübingen Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran. Medicine Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina Kai Januschowski1, Sebastian Müller1, Carlo Krupp1, Martin S. Spitzer1, José Hurst1, Maximilian Schultheiss1, Karl-Ulrich Bartz-Schmidt1, Peter Szurman1, Sven Schnichels1 1Centre for Ophthalmology, University Eye Hospital Tübingen With this study, we introduce a standardized stress model for the isolated superfused bovine retina for future preclinical therapeutic testing. The effect of either hypoxia (pure N2) or glutamate stress (250 µM glutamate) on retinal function represented by a- and b-wave amplitudes was evaluated.
