Leibniz Institute for Natural Product Research and Infection Biology Hans Knoll Institute 2 articles published in JoVE Immunology and Infection Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry Susann Hartung1,2, Christopher Rauh2, Sarah Böttcher1, Mai Thi Ngoc Hoang1,2, Susanne Jahreis1,2, Silke Rummler3, Andreas Hochhaus2, Marie von Lilienfeld-Toal1,2 1Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology, Jena University Hospital, 3Institute for Transfusion Medicine, Jena University Hospital This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes. Cancer Research Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps Fabian L. Kriegel1,2, Ralf Köhler2, Jannike Bayat-Sarmadi2, Simon Bayerl3, Anja E. Hauser2,3, Raluca Niesner2, Andreas Luch*1, Zoltan Cseresnyes*4 1Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), 2Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, a Leibniz Institute, 3Charité Universitätsmedizin Berlin, 4Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Here, we provide a workflow that allows the identification of healthy and pathological cells based on their 3-dimensional shape. We describe the process of using 2D projection outlines based on the 3D surfaces to train a Self-Organizing Map that will provide objective clustering of the investigated cell populations.
