Universitatsklinikum Hamburg - Eppendorf 2 articles published in JoVE Immunology and Infection CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites Julia K. Bialek*1,2, Thomas Walther*1, Joachim Hauber1,3, Ulrike C. Lange1,2,3 1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Department of Anesthesiology, University Medical Center Hamburg-Eppendorf, 3German Center for Infection Research (DZIF) We present a genome engineering workflow for the generation of new in vitro models for HIV-1 infection that recapitulate proviral integration at selected genomic sites. Targeting of HIV-derived reporters is facilitated by CRISPR-Cas9-mediated, site-specific genome manipulation. Detailed protocols for single-cell clone generation, screening, and correct targeting verification are provided. Medicine Prehospital Thrombolysis: A Manual from Berlin Martin Ebinger1,2,5, Sascha Lindenlaub1,3,5, Alexander Kunz1,2,5, Michal Rozanski2,5, Carolin Waldschmidt2,5, Joachim E. Weber2,5, Matthias Wendt2,5, Benjamin Winter1,2,5, Philipp A. Kellner4,5, Sabina Kaczmarek4,5, Matthias Endres1,2,5, Heinrich J. Audebert1,2,5 1Center for Stroke Research Berlin (CSB), Charité - Universitätsmedizin Berlin, 2Klinik und Hochschulambulanz für Neurologie, Charité - Universitätsmedizin Berlin, 3Medical School of the Universität Hamburg, Universitätsklinikum Hamburg - Eppendorf, 4Berliner Feuerwehr, 5STEMO-Consortium Identification of suspected stroke in the dispatch center of the Berlin Fire Brigade prompts the deployment of a CT-equipped ambulance. If ischemic stroke is confirmed and contraindications are excluded prehospital thrombolysis is applied.
