Julius-Maximilians-Universitat Wurzburg 1 article published in JoVE Environment Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms Alexander Marsell1, Christian Fröschel1 1Department of Pharmaceutical Biology, Julius-von-Sachs-Institute, Julius-Maximilians-Universität Würzburg This protocol presents a detailed summary of strategies to inoculate plant roots with soil-borne microbes. Exemplified for the fungi Verticillium longisporum and Verticillium dahliae, three different root infection systems are described. Potential applications and possible downstream analyses are highlighted, and advantages or disadvantages are discussed for each system.
