Hvidovre Hospital 1 article published in JoVE Immunology and Infection A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo Line L. Holm1,2,3, Milica Vukmanovic-Stejic4, Thomas Blauenfeldt1, Thomas Benfield2,3, Peter Andersen1, Arne N. Akbar4, Morten Ruhwald1 1Department of Infectious Disease Immunology, Center for Vaccine Research, Statens Serum Institut, 2Department of Infectious Diseases, Hvidovre Hospital, 3Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 4Division of Infection and Immunity, University College London Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.