Carlsberg Research Laboratory 1 article published in JoVE Biochemistry Determination of the Glycogen Content in Cyanobacteria Alice De Porcellinis1, Niels-Ulrik Frigaard2, Yumiko Sakuragi3 1Carlsberg Research Laboratory, 2Department of Biology, University of Copenhagen, 3Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, University of Copenhagen Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.
