Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) 3 articles published in JoVE Environment Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images José A. Fernandez-Gallego1,2,3, María Luisa Buchaillot1,2, Adrian Gracia-Romero1,2, Thomas Vatter1,2, Omar Vergara Diaz1,2, Nieves Aparicio Gutiérrez4, María Teresa Nieto-Taladriz5, Samir Kerfal6, Maria Dolors Serret1,2, José Luis Araus1,2, Shawn C. Kefauver1,2 1Plant Physiology Section, Faculty of Biology, University of Barcelona, 2Agrotecnio, 3Programa de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Ibagué, 4Instituto Tecnológico Agrario de Castilla y León (ITACyL), 5Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 6Syngenta Spain We present a protocol for counting durum wheat and barley ears, using natural color (RGB) digital photographs taken in natural sunlight under field conditions. With minimal adjustments for camera parameters and some environmental condition limitations, the technique provides precise and consistent results across a range of growth stages. Developmental Biology Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization María Jesús Sánchez-Calabuig1,2, Francisco Alberto García-Vázquez3, Ricardo Laguna-Barraza1, Carlos Barros-García4, Daniel García-Parraga5, Dimitrios Rizos1, Alfonso Gutiérrez Adan1, José Félix Pérez-Gutíerrez2 1Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5 Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes. Biology Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo Dorota N. Komar1, Alfonso Mouriz1, José A. Jarillo1, Manuel Piñeiro1 1Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.
