Universidad de Valencia 3 articles published in JoVE Neuroscience Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations Isabel Mateos-White1, Jaime Fabra-Beser1, David de Agustín-Durán1, Cristina Gil-Sanz1 1Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física, Universidad de Valencia Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest. Biology Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis Jessica Martinez1, Viviana Bisbal2, Nerea Marin2, Antonio Cano1,3,4, Raul Gómez1 1Instituto de Investigación Sanitaria, 2Unidad de Animalario del Centro de Investigación Príncipe Felipe (CIPF), 3Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, 4Servicio de Obstetricia y Ginecología, Hospital Clínico Universitario de Valencia Here, we present a protocol for live-imaging of fluorescently labeled human endometrial fragments grafted in mice. The method allows studying the effects of drugs of choice on endometriotic lesion size through monitoring and quantification of fluorescence emitted by the fluorescent reporter on real time Developmental Biology Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects Eva Porlan1, Beatriz Martí-Prado2,3, Antonella Consiglio4,5, Isabel Fariñas2,3 1Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), 2Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 3Departmento de Biologìa Celular, Universidad de Valencia, 4Institut de Biomedicina de la Universitat de Barcelona (IBUB), 5Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit, University of Brescia Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.
