Parc Cientific de Barcelona 2 articles published in JoVE Developmental Biology Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages Federica Mangione1,2, Enrique Martin-Blanco1 1Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Científic de Barcelona, 2The Francis Crick Institute This protocol is designed for the imaging and analysis of the dynamics of cell orientation and tissue growth in the Drosophila abdominal epithelia as the fruit fly undergoes metamorphosis. The methodology described here can be applied to the study of different developmental stages, tissues, and subcellular structures in Drosophila or other model organisms. Neuroscience Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development Vanessa Gil1,2,3,4, José Antonio Del Río1,2,3,4 1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience, University of Barcelona Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.
