Instituto Agroalimentario de Aragon - IA2 (CITA-Universidad de Zaragoza) 2 articles published in JoVE Developmental Biology Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping Brenda I. Guerrero1,2, Ma. Engracia Guerra3, Javier Rodrigo1,2 1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2(CITA-Universidad de Zaragoza), Instituto Agroalimentario de Aragón - IA2, 3Departamento de Hortofruticultura, Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto de Investigaciones Agrarias Finca La Orden A methodology for the determination of pollination requirements in Japanese plum-type hybrids is described, which combines field- and laboratory-pollinations and observations of pollen tubes under the fluorescence microscopy with the identification of S-genotypes by PCR and the monitoring of flowering for the selection of pollinizers. Developmental Biology Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses Sara Herrera1,2, Jorge Lora3, José I. Hormaza3, Javier Rodrigo1,2 1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), 3Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM La Mayora-UMA-CSIC) We present a methodology to establish the pollination requirements of apricot (Prunus armeniaca L.) cultivars combining the determination of self-(in)compatibility by fluorescence microscopy with the identification of the S-genotype by PCR analysis.
