Universidad Francisco de Vitoria 2 articles published in JoVE Neuroscience Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration María Portela-Lomba1,3, Diana Simón1, Cristina Russo2, Javier Sierra1, María Teresa Moreno-Flores3 1Facultad de CC Experimentales, Universidad Francisco de Vitoria, 2Dept. Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, 3Dept. Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid We present an in vitro model to assess olfactory ensheathing glia (OEG) neuroregenerative capacity, after neural injury. It is based on a coculture of axotomized adult retinal ganglion neurons (RGN) on OEG monolayers and subsequent study of axonal regeneration, by analyzing RGN axonal and somatodendritic markers. Biology Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ Jesús Espada1,2, Elisa Carrasco3, María I. Calvo-Sánchez1,4, Sandra Fernández-Martos1, Juan José Montoya5 1Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, 2 The aim of this protocol is to induce transient in vivo production of nonlethal levels of reactive oxygen species (ROS) in mouse skin, further promoting physiological responses in the tissue.
