Fundacio Banc de Sang i Teixits de les Illes Balears (FBSTIB) 2 articles published in JoVE Bioengineering Labeling of Extracellular Vesicles for Monitoring Migration and Uptake in Cartilage Explants Maria Antònia Forteza-Genestra1,2, Miquel Antich-Rosselló1,2, Francisco Gabriel Ortega1,2, Guillem Ramis-Munar3, Javier Calvo1,2,4, Antoni Gayà1,2,4, Marta Monjo1,2, Joana Maria Ramis1,2 1Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 2Health Research Institute of the Balearic Islands (IdISBa), 3Cellomics Unit, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, 4Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB) Here, we present a protocol to label platelet lysate-derived extracellular vesicles to monitor their migration and uptake in cartilage explants used as a model for osteoarthritis. Bioengineering Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants Miquel Antich-Rosselló1,2, Maria Antònia Forteza-Genestra1,2, Javier Calvo1,2,3, Antoni Gayà1,2,3, Marta Monjo1,2,4, Joana Maria Ramis1,2,4 1Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 2Health Research Institute of the Balearic Islands (IdISBa), 3Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB), 4Department of Fundamental Biology and Health Sciences, University of the Balearic Islands Here, we present a method for the isolation of Extracellular Vesicles (EVs) derived from the platelet lysates (PL) and their use for coating titanium (Ti) implant surfaces. We describe the drop casting coating method, the EVs release profile from the surfaces, and in vitro biocompatibility of EVs coated Ti surfaces.
