Universite de Nantes 3 articles published in JoVE Bioengineering Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes Typhaine Violo1, Christophe Dussouy1, Charles Tellier1, Cyrille Grandjean1, Emilie Camberlein1 1Université de Nantes, CNRS, Unité Fonctionnalité et Ingénierie des Protéines (UFIP), UMR 6286 Genetic code expansion is applied for the introduction of an unnatural amino acid bearing a biorthogonal functional group on a carrier protein at a defined site. The biorthogonal function is further used for the site-selective coupling of a carbohydrate antigen to provide a homogeneous glycoconjugate vaccine. Cancer Research Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation Meriem Bahri*1, Julien Fleurence*1, Sébastien Faraj*1,2, Mohamed Ben Mostefa Daho*1, Sophie Fougeray*1,3, Stéphane Birklé*1,3 1Centre de Recherche en Cancérologie et Immunologie Nantes Angers (CRCINA), Institut national de la santé et de la recherche médicale (INSERM), Université d'Angers, Université de Nantes, Nantes, France, 2Service de chirurgie pédiatrique, Centre hospitalier universitaire (CHU) de Nantes, Nantes, France, 3Unité de Formation et Recherche (UFR) des Sciences Pharmaceutiques et Biologiques, Université de Nantes, Nantes, France This protocol describes how to assess synergism between an anticancer antibody and antineoplastic drugs in preclinical models by using the combination index equation of Chou and Talalay. Immunology and Infection Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood Marie-Claire Devilder1,2,3, Mélinda Moyon1,2,3, Xavier Saulquin1,2, Laetitia Gautreau-Rolland1,2 1CRCINA, Inserm, CNRS, Université d'Angers, Université de Nantes, 2LabEx IGO, "Immunotherapy, Graft, Oncology", 3CHU Nantes, France We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens.
