Institut de Genetique et de Biologie Moleculaire et Cellulaire 4 articles published in JoVE Genetics Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity Tiago Baptista1,2,3,4, Didier Devys1,2,3,4 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France, 2Centre National de la Recherche Scientifique, Illkirch, France, 3Institut National de la Santé et de la Recherche Médicale, Illkirch, France, 4Université de Strasbourg The protocol described here is based on the genome-wide quantification of newly synthesized mRNA purified from yeast cells labeled with 4-thiouracil. This method allows to measure mRNA synthesis uncoupled from mRNA decay and, thus, provides an accurate measurement of RNA polymerase II transcription. Developmental Biology Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo Renee Wei-Yan Chow*1,2,3,4, Paola Lamperti*1,2,3,4, Emily Steed1,2,3,4, Francesco Boselli1,2,3,4, Julien Vermot1,2,3,4 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2UMR7104, Centre National de la Recherche Scientifique, 3U964, Institut National de la Santé et de la Recherche Médicale, 4Université de Strasbourg This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development. Biochemistry Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle Shilpy Joshi1, Vanessa Ueberschlag-Pitiot1, Daniel Metzger1, Irwin Davidson1 1Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire A novel protocol for the preparation of chromatin from adult mouse skeletal muscle adapted to the study of gene regulation in muscle fibers by chromatin immunoprecipitation is presented. Biology Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster Pietro Laneve1,2, Angela Giangrande1 1Département de Génomique Fonctionnelle et Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2Center for Life NanoScience, Istituto Italiano di Tecnologia The aim of this publication is to visualize and discuss the operative steps of an Enhanced Northern Blot protocol on RNA extracted from Drosophila melanogaster embryos, cells, and tissues. This protocol is particularly useful for the efficient detection of small RNA species.
