University of Orleans 2 articles published in JoVE Biochemistry Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein Béatrice Vallée1, Michel Doudeau1, Fabienne Godin1, Hélène Bénédetti1 1Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling. Environment High-pressure, High-temperature Deformation Experiment Using the New Generation Griggs-type Apparatus Jacques Précigout1, Holger Stünitz1,2, Yves Pinquier3, Rémi Champallier1, Alexandre Schubnel3 1Institut des Sciences de la Terre d’Orléans (ISTO), UMR 7327, CNRS-BRGM, Université d’Orléans, 2Department of Geology, University of Tromsø, 3Laboratoire de Géologie, UMR 8538, CNRS, Ecole Normale Supérieure (ENS Paris) Rock deformation needs to be quantified at high pressure. A description of the procedure to perform deformation experiments in a newly designed solid-medium Griggs-type apparatus is here given. This provides technological basis for future rheological studies at pressures up to 5 GPa.
