Genoscope 1 article published in JoVE Chemistry Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling Andrew C. Tolonen1,2,3, Wilhelm Haas4 1CEA, DSV, IG, Genoscope, 2CNRS-UMR8030, Évry, France, 3Université d'Évry Val d'Essonne, 4Massachusetts General Hospital Cancer Center Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a rapid, inexpensive strategy for accurate mass spectrometry-based quantitative proteomics. Here we demonstrate a robust method for preparation and analysis of protein mixtures using the ReDi approach that can be applied to nearly any sample type.
