Institute Paoli-Calmettes 2 articles published in JoVE Biochemistry Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations Mirna Swayden1, Aurélie Dobric1, Yolande Berthois1, Hugo Villalba1, Stéphane Audebert1, Luc Camoin1, Juan Iovanna1, Philippe Soubeyran1 1Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM This protocol aims at establishing ubiquitin (Ub) and ubiquitin-likes (Ubls) specific proteomes in order to identify alterations of these kind of post-translational modifications (PTMs), associated with a specific condition such as a treatment or a phenotype. Medicine Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation Carmen Mariana Aanei1, Marie-Christine Jacob2, Richard Veyrat-Masson3, Tiphanie Picot1, Maria Alessandra Rosenthal-Allieri4, Anne-Catherine Lhoumeau5, Michel Ticchioni4, Florent Dumezy6, Lydia Campos Catafal1 1Department of Hematology, University Hospital of Saint-Etienne, 2Department of Immunology, University Hospital of Grenoble-Alpes, 3Department Hematology, University Hospital of Clermont-Ferrand, 4Department of Immunology, University Hospital of Nice, 5Department of Biopathology, Institute Paoli-Calmettes, 6Department of Hematology, University Hospital of Lille The MDS diagnosis is difficult in the absence of morphological criteria or non-informative cytogenetics. MFC could help refine the MDS diagnostic process. To become useful for clinical practice, the MFC analysis must be based on parameters with sufficient specificity and sensitivity, and data should be reproducible between different operators.
