Hopitaux Universitaires de Strasbourg 4 articles published in JoVE Biology FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria. Hanna Manko1, Vincent Normant2,3, Quentin Perraud2,3, Tania Steffan1, Véronique Gasser2,3, Emmanuel Boutant1, Éléonore Réal1, Isabelle J. Schalk2,3, Yves Mély1, Julien Godet1,4 1Université de Strasbourg, Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021, 2Université de Strasbourg, UMR 7242, ESBS, 3CNRS, UMR 7242, ESBS, 4Groupe Méthode Recherche Clinique, Hôpitaux Universitaires de Strasbourg We describe here a protocol to characterize protein-protein interactions between two highly-differently expressed proteins in live Pseudomonas aeruginosa using FLIM-FRET measurements. The protocol includes bacteria strain constructions, bacteria immobilization, imaging and post-imaging data analysis routines. Medicine The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice Ipek Yalcin1, Salim Megat1,2, Florent Barthas1,2, Elisabeth Waltisperger1, Mélanie Kremer1,2, Eric Salvat1,2,3, Michel Barrot1 1Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique, 2Université de Strasbourg, 3Hôpitaux Universitaires de Strasbourg Neuropathic pain is a consequence of a lesion or disease affecting the somatosensory system. The “cuff model” of neuropathic pain in mice consists of the implantation of a polyethylene cuff around the main branch of the sciatic nerve. Mechanical allodynia is tested using von Frey filaments. Medicine In vivo Macrophage Imaging Using MR Targeted Contrast Agent for Longitudinal Evaluation of Septic Arthritis Guillaume Bierry1,2, Sophie Lefevre2,3, Jean-Louis Dietemann1, François Jehl2,3 1Department of Radiology, University Hospital of Strasbourg, 2EA 3432, University of Strasbourg, 3Department of Bacteriology, University Hospital of Strasbourg We demonstrate how to perform macrophage MR imaging using ultrasmall superparamagnetic contrast agent (USPIO) in septic arthritis, allowing an initial and longitudinal in vivo non-invasive evaluation of macrophages infiltration and an assessment of therapy efficacy. Bioengineering Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo Nihal E. Vrana1, Agnes Dupret-Bories1,2, Christophe Chaubaroux1, Elisabeth Rieger1,2, Christian Debry1,2, Dominique Vautier1,3, Marie-Helene Metz-Boutigue1,3, Philippe Lavalle1,3 1Biomatériaux et Bioingénieriee, INSERM, 2Service Oto-Rhino-Laryngologie, Hôpitaux Universitaires de Strasbourg, 3Faculté de Chirurgie Dentaire, Université de Strasbourg In this video, we will demonstrate modification techniques for porous metallic implants to improve their functionality and to control cell migration. Techniques include development of pore gradients to control cell movement in 3D and production of basement membrane mimics to control cell movement in 2-D. Also, a HPLC-based method for monitoring implant integration in-vivo via analysis of blood proteins is described.
