University of Artois 1 article published in JoVE Biology A High Output Method to Isolate Cerebral Pericytes from Mouse Anupriya Mehra1, Lucie Dehouck1, Elodie Vandenhaute1, Marc Fatar2, Laurence Fenart1, Fabien Gosselet1 1Laboratory of the Blood Brain Barrier, University of Artois, 2Department of Neurology, Universitätsmedizin Mannheim, Heidelberg University We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.